recombinant cfah  (R&D Systems)

Journal: Cellular and Molecular Neurobiology

Article Title: Fibrinogen and Complement Factor H Induce Parkinsonian and Cognitive Impairment-Like Features in Mice

doi: 10.1007/s10571-025-01576-8

Figure Lengend Snippet: A Representative paw-print impressions of control, Fib 2.5, and CFAH 5 on the catwalk gait analysis system. The bottom panel shows the shortening and drag in the prints of CFAH group. B Histograms showing mean stride length of RF, RH, LF, and LH. RF shows a significant reduction in the mean stride length (Fib 2.5 vs. control * p = 0.0432; CFAH 5 vs. control * p = 0.0170). There are no significant changes in the stride length of RH, LF, and LH

Article Snippet: 2.5 μg and 5 μg of native fibrinogen (ab233609 AbCam UK) and recombinant CFAH (4999-FH-050 R & D systems) followed by observation of the effects, 48 h post-injection.

Techniques: Control

Journal: Cellular and Molecular Neurobiology

Article Title: Fibrinogen and Complement Factor H Induce Parkinsonian and Cognitive Impairment-Like Features in Mice

doi: 10.1007/s10571-025-01576-8

Figure Lengend Snippet: Histograms depicting different parameters of the NOR test with mean ± SEM. A Note the trend of reduction in the discrimination index (DI) of Fib 2.5 and CFAH 5 groups. B No significant change observed in the percentage of time spent with novel object. C Significant change observed in the percentage of time spent with familiar object (Fib 2.5 vs. vehicle * p = 0.0299). D Exploration of other zones (non-exploration) where the CFAH 5 group shows a significantly low exploration (CFAH 5 vs. vehicle *p = 0.0145). E In the overall exploration time, the CFAH 5 group shows a significantly low percentage (CFAH 5 vs. vehicle * p = 0.0205)

Article Snippet: 2.5 μg and 5 μg of native fibrinogen (ab233609 AbCam UK) and recombinant CFAH (4999-FH-050 R & D systems) followed by observation of the effects, 48 h post-injection.

Techniques:

Journal: Cellular and Molecular Neurobiology

Article Title: Fibrinogen and Complement Factor H Induce Parkinsonian and Cognitive Impairment-Like Features in Mice

doi: 10.1007/s10571-025-01576-8

Figure Lengend Snippet: Representative low-magnification photomicrographs of TH immunopositive DA neurons within SNpc of A control, B Fib 2.5, C Fib 5, D CFAH 2.5, and E CFAH 5. F The histogram shows a significant reduction in the number of DA neurons in Fib 2.5 in comparison to vehicle Fib 2.5 vs. vehicle * p = 0.0224, G The number of degenerating DA neurons is more in the CFAH 2.5 vs. vehicle * p = 0.0266. H A significant increase in the soma size of DA neurons in Fib 5 µg (Fib 5 vs. vehicle * p = 0.0128) and CFAH 5 µg (CFAH 5 vs. vehicle * p = 0.0483) injected mice. Scale bar = 250 µm for all micrographs

Article Snippet: 2.5 μg and 5 μg of native fibrinogen (ab233609 AbCam UK) and recombinant CFAH (4999-FH-050 R & D systems) followed by observation of the effects, 48 h post-injection.

Techniques: Control, Comparison, Injection

Journal: Cellular and Molecular Neurobiology

Article Title: Fibrinogen and Complement Factor H Induce Parkinsonian and Cognitive Impairment-Like Features in Mice

doi: 10.1007/s10571-025-01576-8

Figure Lengend Snippet: Representative photomicrographs of VTA-DA neurons showing cellular TH expression in A vehicle, B Fib 2.5, C Fib 5, D CFAH 2.5, and E CFAH 5-administered study groups. A1, C1, E1 Photomicrographs show VTA at 100X from control, Fib 5 and CFAH 5. F Histogram show significant reduction in cytoplasmic TH expression in Fib 2.5 vehicle * p = 0.421). G Histogram showing significant increase in the cytoplasmic area (Fib 5 vs. vehicle * p = 0.0364). Representative photomicrographs of nigral DA neurons showing cellular TH expression of the medial subregion in H vehicle, I Fib 2.5, and J Fib 5. J1a Arrow denotes degenerating neurons and J1b Arrowhead points toward a cytoplasmic vacuole in Fib 5. TH expression in nigral DA neurons in K CFAH 2.5 and L CFAH 5. M Medial SN showed no significant change in TH expression and N cytoplasmic area. Scale bar = 50 µm for all micrographs

Article Snippet: 2.5 μg and 5 μg of native fibrinogen (ab233609 AbCam UK) and recombinant CFAH (4999-FH-050 R & D systems) followed by observation of the effects, 48 h post-injection.

Techniques: Expressing, Control

Journal: Cellular and Molecular Neurobiology

Article Title: Fibrinogen and Complement Factor H Induce Parkinsonian and Cognitive Impairment-Like Features in Mice

doi: 10.1007/s10571-025-01576-8

Figure Lengend Snippet: Representative photomicrographs of DA neurons of the dorsal ( A – E , A1 – C1 ) and lateral ( H – L ; H1 – K1 ) sub-regions of the SNpc, showing pan-cytoplasmic TH expression. The alterations are noted in the dorsal region ( A – E , A1 – C1 ) of the vehicle-injected mice. A A single healthy DA neuron at higher magnification is shown in (100X, A1 ). The neurons of the fibrinogen- and CFAH-injected mice are shown in ( B–E) . B Fib 2.5, B1 **represent smaller neurons, C Fib 5, C1 Arrow heads denote degenerating neurons in Fib 5. D . CFAH 2.5 E CFAH 5. F Histograms showing significant reduction in TH expression in the fibrinogen-injected group (Fib 5 vs. vehicle ** p = 0.0188). G **Asterisks denote the significant decrease/shrinkage in cytoplasmic area of dorsal neurons (Fib 2.5 vs. vehicle *** p = 0.0008). Representative photomicrographs of the DA neurons of the lateral subregion ( H – L ) of the SNpc showing cellular TH expression in H vehicle; I Fib 2.5; J Fib 5; K CFAH 2.5 and L CFAH 5. H1 . Photomicrograph showing a single healthy DA neuron at higher magnification (100X); J1 Photomicrographs (100X) showing degenerating DA neuron at higher magnification in Fib 5 and K1 CFAH 2.5. M Histograms show a significant reduction in TH in the fibrinogen-injected group Fib 5 vs. vehicle * p = 0.0259. N Shrinkage is seen in the cytoplasmic area (Fib 2.5 vs. vehicle * p = 0.0428). Scale bar = 50 µm for all micrographs

Article Snippet: 2.5 μg and 5 μg of native fibrinogen (ab233609 AbCam UK) and recombinant CFAH (4999-FH-050 R & D systems) followed by observation of the effects, 48 h post-injection.

Techniques: Expressing, Injection

Journal: Cellular and Molecular Neurobiology

Article Title: Fibrinogen and Complement Factor H Induce Parkinsonian and Cognitive Impairment-Like Features in Mice

doi: 10.1007/s10571-025-01576-8

Figure Lengend Snippet: Representative photomicrographs of the striatum (entire striatum) showing TH expression in the DA terminals across various groups. The upper panel shows low-magnification (4X) images of different study groups, i.e., A vehicle, B Fib 2.5, C Fib 5, D CFAH 2.5, and E CFAH 5. The lower panel shows high magnification (40X) images of the dorsolateral striatum (DL) different study groups, i.e., F vehicle, G Fib 2.5, H Fib 5, I CFAH 2.5, and J CFAH 5. K Histograms showing differential expression of TH in terms of OD in the striatum (Fib 5 vs. vehicle * p = 0.0110). L Dorsolateral striatum (DL) shows a decrease in the expression (Fib 5 vs. vehicle ** p = 0.0092). M No significant changes are seen in striatal volume. Scale bar of all micrographs = 500 µm

Article Snippet: 2.5 μg and 5 μg of native fibrinogen (ab233609 AbCam UK) and recombinant CFAH (4999-FH-050 R & D systems) followed by observation of the effects, 48 h post-injection.

Techniques: Expressing, Quantitative Proteomics

Journal: Cellular and Molecular Neurobiology

Article Title: Fibrinogen and Complement Factor H Induce Parkinsonian and Cognitive Impairment-Like Features in Mice

doi: 10.1007/s10571-025-01576-8

Figure Lengend Snippet: Representative low-magnification photomicrographs of mice CA1 following different injection regimes for the proteins, i.e., fibrinogen and CFAH: A Vehicle, B Fib 2.5, and C Fib 5. C1 Arrows indicate chromatolysis, D CFAH 2.5. E CFAH 5. E ****(asterisks) represent necroptotic cells. Histograms showing the morphometric index of CA1 neurons. F Histograms show significant increase in the area of the neuronal nucleus of Fib 5 µg, (Fib 5 vs. vehicle ** p = 0.0026). G A significant increase in the cytoplasmic area of the neurons Fib 2.5 vs. vehicle ** p = 0.0090, Fib 5 vs. vehicle * p = 0.0182. H Histogram showing unaltered volume of the CA1. I Vehicle- J Fib 2.5-, K Fib 5-, and L CFAH 2.5-injected animals showed patches of hypertrophied cells. M Arrows denote the ‘necroptotic spots’ in the subiculum of CFAH 5 study group, indicating degenerative changes. N The histograms confirm nuclear enlargement of the neuronal nucleus of Fib 2.5 µg, Fib 5 µg, CFAH 5 µg, Fib 2.5 vs. vehicle * p = 0.0461, Fib 5 vs. vehicle ** p = 0.0012, CFAH 5 vs. vehicle * p = 0.0376). O Histograms showing significant increase in cytoplasmic area in Fib 2.5 and Fib 5 study groups, Fib 2.5 vs. vehicle * p = 0.0114, Fib 5 vs. vehicle ** p = 0.0063) both suggesting cellular hypertrophy. P Histogram showing unaltered volume. Scale bar = 10 µm

Article Snippet: 2.5 μg and 5 μg of native fibrinogen (ab233609 AbCam UK) and recombinant CFAH (4999-FH-050 R & D systems) followed by observation of the effects, 48 h post-injection.

Techniques: Injection

Journal: Cellular and Molecular Neurobiology

Article Title: Fibrinogen and Complement Factor H Induce Parkinsonian and Cognitive Impairment-Like Features in Mice

doi: 10.1007/s10571-025-01576-8

Figure Lengend Snippet: Representative low-magnification photomicrographs of mice colon following vehicle ( A – A2 ) and different injection regimes for fibrinogen ( B – B2 and C – C1 ) and CFAH ( D – D2 and E – E1 ) at different magnifications (4× and 10×). B Fib 2.5. C Fib 5 ( C1 ). Hashes denote the presence of inflammatory patches (##; B , B1 , B2 , C1 , E1 ) in the guts of mice injected with either Fib or CFAH. F The cryptic length appears unaltered. G Histograms show the presence of significantly higher number of folds in colonic lumen of the CFAH-injected group (CFAH 2.5 vs. vehicle ** p = 0.0046) and H the considerable thickening of the Tunica muscularis in the CFAH-injected group (CFAH 2.5 vs. vehicle ** p = 0.0059)

Article Snippet: 2.5 μg and 5 μg of native fibrinogen (ab233609 AbCam UK) and recombinant CFAH (4999-FH-050 R & D systems) followed by observation of the effects, 48 h post-injection.

Techniques: Injection